Fig. 5.

ESI-LCMS data for modified peptides isolated from the TclN-defective strain after shorter (8 h, Top) or longer (16 h, Bottom) culture times. Decarboxylated peptide series (green peaks) and nondecarboxylated series (red peaks) display within-series mass differences (deconvoluted) of 18 amu. Inset table reports deconvoluted masses of peaks a–i and the degree of dehydration (3x–8x) for each peak. Subtle isotopic details (d, Insets) provide additional information about peptide processing (see text), including a minor amount of thiazole formation (#) and a shift in the most abundant isotope (*).