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. 2016 Oct 17;113(44):12426–12431. doi: 10.1073/pnas.1612745113

Fig. 1.

Fig. 1.

Py-Im polyamides are strong roadblocks for RNA pol II transcription elongation. (A) Four Py-Im polyamides share the same 6-bp DNA target sequence (5′-WGGWCW-3′). The black solid circle refers to N-methylimidazole (Im), and the hollow circle refers to N-methylpyrrole (Py). (B) RNA pol II transcription blockage by Py-Im polyamides with a TS binding orientation and (C) with a NTS binding orientation. (D and E) PAGE gel analysis of TFIIS effect on Py-Im polyamide induced pol II arrest. The Py-Im polyamide binds DNA in a TS orientation (D) or NTS orientation (E). In these gel images, control lanes are RNA pol II transcription in the absence of Py-Im polyamides. Time points for transcription assays were 10 min, 20 min, 30 min, 1 h, and 2 h. The concentration of Py-Im polyamides was 0.5 µM. (F) Quantitative analysis of TFIIS effect on rescuing Py-Im polyamide-induced pol II arrest.