Fig. 2.

Py-Im polyamides cause sequence-specific inhibition of pol II transcription elongation. The Py-Im polyamide binding site is in a TS orientation. (A) Scheme of single mutation of the designed target sequence. The PM sequence is 5′-WGGWCW-3′, and the single-base MM sequence is 5′-WGWWCW-3′ (mutation underlined). (B) Comparison of inhibition of 1 on pol II transcription elongation from the PM or MM sequence. The effect of TFIIS was also investigated as shown in PAGE gel. Time points were 10 min, 20 min, 30 min, 1 h, and 2 h. Py-Im polyamide concentration was 0.5 µM. (C) Quantitative analysis of selective transcriptional inhibition by 1.