Fig. S6.

Py-Im polyamide cause sequence-specific inhibition of pol II transcription elongation. The polyamide binding site is in a NTS orientation. (A) Scheme of single mutation of the designed target sequence. The PM sequence is 5′-WGGWCW-3′, whereas the single base MM sequence is 5′-WGWWCW-3′ (mutation underlined). (B) Comparison of inhibition of polyamide 1 on pol II transcription elongation from the PM sequence or MM sequence. The effects of TFIIS were also investigated as shown in PAGE gel. Time points were 10 min, 20 min, 30 min, 1 h, and 2 h. Py-Im polyamide concentration was 0.5 µM. (C) Quantitative analysis of selective transcriptional inhibition by polyamide 1.