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. 2016 Nov 7;11(11):e0165831. doi: 10.1371/journal.pone.0165831

Fig 2. Effect of GRA and GRA derivatives on S. aureus MW2 growth.

Fig 2

Overnight cultures of S. aureus MW2 were adjusted to an OD of 1.0. Then, 100 μl of bacterial culture was inoculated into 5 ml of TSB. The bacterial culture was incubated at 37°C with shaking. When the OD at 660 nm reached 0.3, various concentrations (■: control, ♦: 1/64 MIC, ▲: 1/16 MIC, ●: 1/4 MIC, ×: 1x MIC, +: 2x MIC) of GR-SU or GRA were added to the medium. The growth and colony counts were monitored. Three independent experiments were performed, and the mean ± SD was calculated. The data were analyzed for statistically significant differences compared to untreated control by a two-way ANOVA followed by Dunnett’s post hoc tests. *P<0.05.