Table 2. Summary of Env mutations that affect trimer conformations.

Env region	Substitution	Specificity	Affected Env region
			CD4bs	V3 loop 1	TAD V2q epitope 2	2G12 glycans
V2 loop	160N	Only LN40 studied	more exposed to sCD4 but not CD4bs mabs	more exposed	not tested	not tested
CD4 binding loop, N-terminal flank	362D	LN8 specific	mab b12 epitope more exposed	hidden	no effect	shifted
	363D	LN40 specific	mab b12 epitope more exposed	more exposed	not tested	shifted
	365V	Z1792M, LN8 more marginal	No effect	still hidden	enhanced presentation	no change
Phe-43 cavity	375W	cross clade 3	more exposed	still hidden	reduced	shifted
CD4 binding loop, C-terminal flank	377V	cross clade 3	more exposed	exposed	reduced	shifted
	380P	cross clade 3	more exposed	highly exposed	reduced	shifted

1 Not tested on Z1792M.

2 Not tested on LN40

3 V3 exposure not tested on clade C, Z1792M