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. 2016 Nov 7;11(11):e0165078. doi: 10.1371/journal.pone.0165078

Fig 1. NAP1-assisted nucleosome assembly.

Fig 1

(a) Schematic diagram of a tethered DNA molecule under tension in the magnetic tweezers assay. Torsionally unconstrained (nicked) DNA molecules were selected for nucleosome assembly. Assembly of a nucleosome results in shortening of the end-to-end length of the DNA. (b) Real-time observation of nucleosome assembly at 0.5 pN. Thin blue line shows raw data at 50 Hz bandwidth and thick blue line is the moving average with 1 s time window. During the flow of a buffer carrying histones and NAP1, the force was increased to 12 pN to suppress non-specific binding of the bead to the surface. After the flow, the force was lowered again to 0.5 pN and shortening of the DNA lengths was observed as nucleosomes assemble.