Figure 1.

Contribution of T cells to paclitaxel-induced mechanical allodynia. A, Paclitaxel (2 mg/kg, i.p.) was administered to WT (C57BL/6) and Rag1^−/− mice on day 0 and day 2. Mechanical allodynia was measured using von Frey hairs and the 50% paw withdrawal threshold was calculated using the up-and-down method. WT + vehicle (circles, gray line); Rag1^−/− + vehicle (open squares, gray dashed line); WT + paclitaxel (diamonds black line); and Rag1^−/− + paclitaxel (open triangles, black dashed line). Two-way repeated-measures ANOVA revealed a main effect of time (p < 0.01), a group effect (p < 0.01), a genotype effect (p < 0.01) and a 3-way interaction among group, genotype, and time (p < 0.01). Bonferroni post hoc analysis showed differences between groups (WT + vehicle vs WT + paclitaxel or Rag1^−/− + vehicle vs Rag1^−/− + paclitaxel) at various time points; n = 8–10/group. **p < 0.01, ***p < 0.001. B, WT and Rag1^−/− mice received PBS or CD3⁺ T cells intravenously 1 d before paclitaxel treatment as in A. Shown are: WT + PBS (circles, gray line); WT + T cells (diamonds, black line); Rag1^−/− + PBS (open triangles, gray dashed line); and Rag1^−/− + T cells (open squares, black dashed line). Two-way repeated-measures ANOVA revealed a main effect of time (p < 0.01), a group effect (p < 0.01), a treatment effect (T cell vs PBS, p < 0.01), and a 3-way interaction among group, treatment, and time (p < 0.01). Bonferroni post hoc analysis showed differences between groups (WT+ PBS vs WT + PBS or Rag1^−/− + PBS vs Rag1^−/− + T cells) at various time points; n = 6–8/group. *p < 0.05, **p < 0.01.