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. 2016 Nov 1;11:5697–5708. doi: 10.2147/IJN.S111274

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© 2016 Duan et al. This work is published and licensed by Dove Medical Press Limited

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Percentage release of calcein from the four liposomes under acidic and physiological pHs.

Notes: Calcein-encapsulated liposomes 4%PEG-HS-CHEM-Cal (A), 1%PEG-HS-CHEM-Cal (B), 2%PEG-HS-CHEM-Cal (C) and 4%PEG-HS-Chol-Cal (D) were tested in buffers of pH 4.5, 5.5 and 7.2 at 37°C. Measurements (n=3) were taken at 30 (initial time point), 60, 90, 120 and 180 min, respectively, into incubation. The release of calcein at each time point is expressed as a percentage of total release (mean% ± SD). Release at 30 min was compared to that of all other time points, and the statistical significance is expressed as follows: ns, P>0.05; *P<0.05; **P<0.001; ***P<0.0001.

Abbreviations: PEG, polyethylene glycol; HS, hydrogenated soy; CHEM, cholesteryl hemisuccinate; Cal, calcein; Chol, cholesterol; ns, not significant; SD, standard deviation.