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. 2016 Nov 8;6:234. doi: 10.3389/fonc.2016.00234

Figure 3.

Figure 3

RNA ISH specificity test. RNA ISH specificity test with DAPI, CK 8, 18, 19-type 4, HER2 sense/anti-sense probes, and EGFR sense/anti-sense probes. The parallel stained cells are the NME cells from healthy donor spiked with BT474 cells at a ratio of 4:1 in cell number. (1)–(4) are the slide with DAPI, CK 8, 18, 19-type 4, EGFR-type 1; (5)–(8) are the slide with DAPI, CK 8, 18, 19-type 4, sense-EGFR-type 1. (9)–(12) are the slide with DAPI, CK 8, 18, 19-type 4, HER2-type 6. (13)–(16) are the slide with DAPI, CK 8, 18, 19-type 4, sense-HER2-type 1.