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Integrated RNA ISH and ICC staining on Hedgehog pathway markers. RNA ISH staining with Smo-type 1 and Gli1-type 6. (A) is the RNA ISH staining of T47D cell line with CK 8, 18, 19-type 4, Smo-type 1, and Gli1-type 6. (B) is the integrated RNA ISH and fluorescent ICC staining with CK-AF488 (ICC), Smo-type 1 (RNA ISH), and Gli1-type 6 (RNA ISH) on the NME cells from a breast cancer patient. CK+, Smo+, and Gli1+ cells are found among these NME cells in the staining.
