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Integrated RNA ISH and ICC staining consistency test. RNA ISH with fluorescent ICC staining with EGFR, HER2 antibody, and EGFR, HER2 RNA ISH on blood cells spiked with BT474 cells at a ratio of 4:1 in cell number. (A) is the staining of DAPI, EGFR-AF488 (ICC), EGFR-type 1 (RNA ISH), and HER2-type 6 (RNA ISH). (B) is the staining of DAPI, HER2-AF488 (ICC), EGFR-type 1 (RNA ISH), and HER2-type 6 (RNA ISH).
