Table 1.
Sequence-specific nucleases designed to engineer virus resistance.
| Nuclease | Virus | Plant | Target | References |
|---|---|---|---|---|
| Zinc finger nuclease (ZFN) | TYLCCNV and TbCSV | Nicotiana benthamiana | Rep | Chen et al., 2014 |
| TYLCV | Arabidopsis thaliana | Rep binding site in IR | Mori et al., 2013 | |
| TYLCV | A. thaliana | Rep binding site in IR | Koshino-Kimura et al., 2009 | |
| TYLCV | A. thaliana | Rep binding site in IR | Koshino-Kimura et al., 2008 | |
| TYLCV | A. thaliana | Rep binding site in IR | Takenaka et al., 2007 | |
| BSCTV | A. thaliana | Rep binding site in IR | Sera, 2005 | |
| Transcription activator-like effector nuclease (TALEN) | TbCSV, TLCYnV, TYLCCNV, and TYLCCNB | N. benthamiana | Rep and Hairpin in IR | Cheng et al., 2015 |
| Clustered regularly interspaced palindromic repeats/CRISPR-associated 9 (CRISPR/Cas9) | TYLCV, BCTV, and MeMV BeYDV BSCTV CLCuKoV, TYLCV 2.3, TYLCSV, MeMV, BCTV-Logan, BCTV-Worland TuMV CVYV, ZYMV, and PRSMV |
N. benthamianaN. benthamiana N. benthamiana and A. thaliana N. benthamiana A. thaliana Cucumis sativus |
IR, CP, and Rep LIR and Rep/RepA IR, CP, and Rep IR, CP, and Rep Host factor eIF(iso)4E Host factor eIF4E |
Ali et al., 2015a Baltes et al., 2015 Ji et al., 2015 Ali et al., 2016 Pyott et al., 2016 Chandrasekaran et al., 2016 |
Tomato yellow leaf curl China virus (TYLCCNV), Tobacco curly shoot virus (TbCSV), Tomato yellow leaf curl virus (TYLCV), Beet severe curly top virus (BSCTV), Tomato leaf curl Yunnan virus (TLCYnV), Tomato yellow leaf curl China betasatellite (TYLCCNB), Beet curly top virus (BCTV), Merremia mosaic virus (MeMV), Bean yellow dwarf virus (BeYDV), Cotton leaf curl Kokhran virus (CLCuKoV), Tomato yellow leaf curl Sardinian virus (TYLCSV), Turnip mosaic virus (TuMV), Cucumber vein yellowing virus (CVYV), Zucchini yellow mosaic virus (ZYMV), Papaya ring spot mosaic virus (PRSMV), replication associated protein (Rep), intergenic region (IR), long intergenic region (LIR), coat protein (CP).