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. 2016 Nov 8;8:270. doi: 10.3389/fnagi.2016.00270

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Copyright © 2016 Brewton, Kokash, Jimenez, Pena and Razak.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Representative examples of perineuronal net (PNN+) and parvalbumin (PV)/PNN co-localized cells are shown. PNN+ cells (green channel) were identified by their complete or near complete ring structure, with relatively less intense staining in the area corresponding to the soma. PV+ cells (red channel) were identified by a staining pattern throughout most of the soma. PNN+ cells without PV are marked with arrowheads. PV+/PNN+ co-localized cells are indicated with a solid arrow. These labels are preserved when the images are separated out into the two channels and when the images are merged. The scale bar is 50 μm and applies to all images.