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. 2016 Nov 8;7:1733. doi: 10.3389/fmicb.2016.01733

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Copyright © 2016 Canovas, Baldry, Bojer, Andersen, Gless, Grzeskowiak, Stegger, Damborg, Olsen and Ingmer.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Modulation of Staphylococcus aureus virulence gene expression by staphylococcal culture supernatants. TSA agar plates (with erythromycin and X-gal) containing (A) the hla-lacZ (PC322; Ery^r), (B) the rnaIII-lacZ (SH101F7; Ery^r), or (C) the spa-lacZ (PC203; Ery^r) reporter strain of S. aureus were exposed to 20 μL (in pre-drilled wells) of supernatants from centrifugation (8000 rpm for 60 s) of overnight cultures of strains 27472 (Staphylococcus intermedius), 28993 (Staphylococcus haemolyticus), 30755 (Staphylococcus pseudintermedius), 30743 (Staphylococcus schleiferi), 29886 (Staphylococcus delphini), 30106 (Staphylococcus warneri) and 2898 (Staphylococcus schleiferi). H₂O was used as a control. Zones appeared between 9 and 36 h of incubation at 37°C. This figure is representative of one set of screening plates.