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. 2016 Nov 8;7:1733. doi: 10.3389/fmicb.2016.01733

FIGURE 4.

FIGURE 4

Staphylococcus schleiferi AIP interferes with S. aureus agr. (A) RNAIII expression was recorded as β-lactamase expressed from the P3-blaZ reporter fusion in S. aureus RN10829(P2-agrA:P3-blaZ)/pagrC-I (WT) with addition of 5% AIP-I supernatant and 20% supernatant from either strain 8325-4Δagr/pRAB12-agrBDSs (AIPSs) or the control strain 8325-4Δagr/pRAB12-lacZ (vector control) that had been grown and induced (0.2 μg/ml anhydrotetracycline) overnight. Each bar represents the average of three biological replicates and the error bars represent the standard deviation. (B) P3-blaZ expression recorded from S. aureus RN10829(P2-agrA:P3-blaZ)/pagrC-I (WT) when the inducing AIP-I containing supernatant (10%) is challenged for 45 min with different concentrations of synthetic S. schleiferi AIP at indicated concentrations. No induction and AIP-I containing supernatant alone was included as controls. Each bar represents the average of three biological replicates and the error bars represent the standard deviation.