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. 2016 Oct 8;6(3):230–240. doi: 10.1016/j.ijpddr.2016.10.002

Fig. 1.

Fig. 1

Regression analysis of pyrosequencing assays for Trichostrongylus isotype 1 (A) and isotype 2 (B) β-tubulin assays for codons F167Y (TTC to TAC), E198A (GAA to GCA) and F200Y (TTC to TAC). Artificial mixtures of plasmids containing the target sequences as templates were mixed and analysed by pyrosequencing. Observed frequencies (mean of 3–5 replicates ± SEM) were plotted against calculated input frequencies. Regression plots (including linear equation and Pearson correlation coefficient) with 95% confidence bands are shown.