Frequency of single nucleotide polymorphisms associated with benzimidazole resistance in isotype 1 β-tubulin in codons F167Y (TAC), E198A (GCA) and F200Y (TAC) in 2012 (A) and 2013 (B). Values represent means of two pyrosequencing assays per farm. The data are shown separately according to country (CH, Switzerland; IT, Italy; IRE, Ireland) and parasite (Hae, Haemonchus spp., Tel, Teladorsagia spp., Tri, Trichostrongylus spp.). The black line represents the overall mean.