Steady state levels of ferritin H in brain tissue of young, adult and old C57BL/6J mice. In panel (A) representative immunohistochemistry of the distribution of ferritin H in young and old murine brain sections is shown. Panel (B) displays the protein expression of ferritin H in young, adult and old murine brain tissues from C57BL/6J male mice. The immunoblots are shown for four individual mice per age group. All data are protein levels in relation to GAPDH, setting the young control at 100%, statistical significant differences between young, adult and old murine brain tissue were calculated, using one-way ANOVA, followed by Tukey's post hoc test and are shown by **p<0.01, all compared to young control.