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. 2016 Sep 12;65(12):1451–1463. doi: 10.1007/s00262-016-1892-8

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 2 — Representative examples of the proliferation and cytokine production of three LNMC cultures. The 22-day cultured LNMC were subjected to a 3-day proliferation assay to determine the response to HPV16 E6 and/or E7 22-mer peptides. Unloaded autologous monocytes served as negative control. The proliferative response is given as the stimulation index (SI) when stimulated with peptide- or protein-loaded autologous monocytes and is displayed on the left y-axis. The supernatant harvested after 48 h of stimulation was used for cytokine analysis by CBA, which is shown in the right y-axis