Table 1.
Primers used for the rabbit (Oryctolagus cuniculus) TLR-1 cloning and expression analyses
| Primer | Accession no. | Sequence (5′ to 3′) | Primer type or use |
|---|---|---|---|
| GAPDH Sense primer | L23961 | GAATCCACTGGCGTCTTCAC | Housekeeping gene primers |
| GAPDH Anti-sense primer | CGTTGCTGACAATCTTGAGAGA | ||
| d-rTLR-1 Forward | – | ATGCTGTAGTAGGCTCAG | Degenerative primers |
| d-rTLR-1 Reverse | – | ATTCATCTGCGTGGTCAT | |
| r-rTLR-1 Forward | KC349941 | TATAAACCAATTTTGCAGTAGTACGCT | RACE primers |
| r-rTLR-1 Reverse | TGGGAGGTAAACATCTGAAAACAGAGTC | ||
| nr-rTLR-1 Forward | AGTCTTTCCCAAGGTAGCATCTACG | ||
| nr-rTLR-1 Reverse | CGTGTCCGTCAAGAGGTTGTTGG | ||
| q-rTLR-1 Forward | AATGCGTTTGATGCTCTGCC | Tissue expression primers | |
| q-rTLR-1 Reverse | CCTCCGTGCCATGAGGGTTT | ||
| rTLR-1 Forward | TGACCATCACTATTTCTAGCAGCTT | Full length primers | |
| rTLR-1 Reverse | CCATGTTTAGTCTTCTCCTTCGGC |