Table 2. Primer sets used in the study.
| Forward primer (5′–3′) | Reversed primer (5′–3′) | |
|---|---|---|
| Genes for genotyping | ||
| Floxed Metrnl (Metrnlloxp/loxp) | TGAGGGTTGGAGGCTCCTAGC | GGATGAGCGTTTGAGCACAGC |
| Cre (Villin-Cre) | GCGGTCTGGCAGTAAAAACTATC | GTGAAACAGCATTGCTGTCACTT |
| Genes for real-time PCR | ||
| Metrnl | CTGGAGCAGGGAGGCTTATTT | GGACAACAAAGTCACTGGTACAG |
| GAPDH | GTATGACTCCACTCACGGCAAA | GGTCTCGCTCCTGGAAGATG |
| Villin1 | TCAAAGGCTCTCTCAACATCAC | AGCAGTCACCATCGAAGAAGC |
| Mucin2 | TGTGTTCACGGGAATGCTGAG | TGCAGGCGATGACGTTGAG |
| SAA3 | TGCCATCATTCTTTGCATCTTGA | CCGTGAACTTCTGAACAGCCT |
| Reg | ATGCTTCCCCGTATAACCATCA | GGCCATATCTGCATCATACCAG |
| Reg3b | ACTCCCTGAAGAATATACCCTCC | CGCTATTGAGCACAGATACGAG |
| Lactotransferrin | TGAGGCCCTTGGACTCTGT | ACCCACTTTTCTCATCTCGTTC |
| Haptoglobin | GCTATGTGGAGCACTTGGTTC | CACCCATTGCTTCTCGTCGTT |
| S100A8 | AAATCACCATGCCCTCTACAAG | CCCACTTTTATCACCATCGCAA |
SAA3, serum amyloid A-3; Reg3g, regenerating islet-derived 3gamma; Reg3b, regenerating islet-derived 3beta; S100A8, S100 calcium binding protein A8.