Figure 6.

Activation of γδ T cells as a result of DLE-associated CD11c⁺ NK cell emergent production. γδ T cells from healthy donors were cocultured with the DLE-derived CD11c⁺ NK cell at a ratio of 2 : 1 CD56⁺CD11c⁺ NK : γδ T cell (a). Both NK and γδ T cell populations were highly purified by flow cytometry sorting before setting the proliferation assay (b). γδ T lymphocyte proliferation was assessed at 72 hours by dilution of Cell Trace Violet (CTV) dye (c).