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. 2016 Oct 25;2016:7694385. doi: 10.1155/2016/7694385

Figure 3.

Figure 3

NRG1 functions through DISC1 to regulate cortical inhibitory neuron dendrite growth. (a) Representative images of DIV21 cortical inhibitory neurons cotransfected with PG67-GFP and PG67-DISC1FL or PG67-DISC1DN on DIV7. Cells were treated with PBS (top panels) or NRG1β (bottom panels) for 2 days. Cultures were stained for GFP to enhance to GFP signal. Images were acquired at 20x. Scale bar = 100 μm. (b) Dendrite growth was analyzed by Sholl analysis using ImageJ. (c) At baseline conditions (PBS treatment), expression of PG67-DISC1DN resulted in a significant decrease in total dendrite growth compared to PG67-GFP controls; expression of PG67-DISC1FL had no effect compared to PG67-GFP controls. In cells treated with NRG1β, both PG67-DISC1FL and PG67-DISC1DN caused a significant decrease in total dendrite growth compared to PG67-GFP controls. Significance determined using a one-way analysis of variance (ANOVA) with Tukey's post hoc tests. Error bars represent standard error of the mean, n = 34–54 cells per condition from 3 experiments; ∗ p < 0.05, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.