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SCA3-iPS cells could differentiate into NSCs and neurons. (a) Immunofluorescence results for Pax6 and Nestin. (b) The flow cytometry analysis below showed that no significant difference was observed in the percentage of Pax6 or Nestin positive cells or Pax6 and Nestin-double positive cells (n = 3). (c) Immunofluorescence results for NeuN and beta-tubulin III. Cell nuclei were stained with DAPI (blue). Scale bars, 20 μm.
