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Analysis of ctDNA from CSF. (A) Allelic discrimination plots and amplification curves of L858R mutation in ctDNA from serum and plasma samples versus CSF, showing a positive amplification only in CSF; (B) allelic discrimination plots and amplification curves of T790M mutation in ctDNA from serum and plasma samples versus CSF, without positive amplification in none of them. CSF, cerebrospinal fluid; ctDNA, circulating tumor DNA.
