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. 2016 Sep 1;3(11):854–865. doi: 10.1002/acn3.347

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© 2016 The Authors. Annals of Clinical and Translational Neurology published by Wiley Periodicals, Inc on behalf of American Neurological Association.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Subcellular localization of intracellularly trapped GJB1 mutants. Confocal fluorescence images of HeLa cells cotransfected with plasmids encoding GJB1 (labeled with a GJB1‐specific antibody; green) and markers of the ER (DsRed‐ER) or the Golgi (DsRed‐Monomer‐Golgi). Similar staining patterns were observed in cells expressing L144del (A), C173Y (B), R183C (C), and E186K (D); GJB1 staining was mainly observed in the perinuclear region and exhibited colocalization with Golgi markers. Cell nuclei were stained with DAPI (blue). Scale bar, 10 μm.