Table 1.
Primers used for reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR).
| Gene | Primer sequences (5′–3′) | Length (bp) | Efficiency (%) | R2 | Linear regression |
|---|---|---|---|---|---|
| 18S | F:CCAGTAAGCGCGAGTCATAA | 103 | 99.6 | 0.9975 | y = -3.3323x + 13.778 |
| R: GGTCCGAAGACCTCACTAAATC | |||||
| 28S | F:TCGAAACGACCTCAACCTATTC | 93 | 113.0 | 0.9869 | y = -3.0462x + 12.955 |
| R: TTGGCACTCTGACCGAAATC | |||||
| 16S | F: GGACCTGCCCACTGAATTATTA | 106 | 97.6 | 0.9992 | y = -3.3805x + 15.749 |
| R: TTCTCATCAAACCATTCATACAAGC | |||||
| EF1A | F: CCTGAAGTGGAAGACGAAGAG | 98 | 106.1 | 0.9782 | y = -3.1839x + 20.008 |
| R: AGAAGGAAAGGCTGATGGTAAA | |||||
| NADH | F: AGTAAGAGGAGTAAAGGCATGAAA | 75 | 91.2 | 0.9961 | y = -3.5523x + 20.036 |
| R:CCTATATGGTTGATTGATGATAAGGC | |||||
| Actin | F: GCGTAACCTTCGTAGATTGGTA | 100 | 102.5 | 0.9994 | y = -3.2646x + 19.117 |
| R: CAAGCTGTACTCTCCCTGTATG | |||||
| Tubulin | F: ACAGGTTTCAAAGTGGGTATCA | 102 | 101.6 | 0.9994 | y = -3.2836x + 23.82 |
| R: GGTGGTGTTTGACAACATGC | |||||
| ArgK | F: GTCGAGCTTAGCCTTGTTAGAG | 103 | 110.6 | 0.9661 | y = -3.091x + 23.19 |
| R: GCTGGGTTTCCTCACTTTCT | |||||
| V-ATPase | F: CCTCAAGGTACACCTCCAATTC | 84 | 91.9 | 0.9935 | y = -3.5346x + 20.23 |
| R: AGCTAATGTTCCAGGACGAATAA |