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. 2016 Nov 8;6:36365. doi: 10.1038/srep36365

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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BALB/c mice were sacrificed 24 hr after ConA or PBS injection. (a–c) Intrahepatic leukocyte was analyzed by flow cytometry. (a) The percentages of NKT cells (CD19⁻CD3⁺αGalCer/CD1d tetramer⁺) were determined, the representative scatterplot (left panel) and summative histogram (right panel) are shown. (b) Measurements of CD69 expression on hepatic NKT cells. (c) The intracellular IFN-γ levels in hepatic NKT cells, the filled flow cytometric histograms represent PBS-treated mice, and the open histograms represent ConA-treated mice. (d) OPN levels in the liver tissue, analyzed by Elisa. Two independent experiments with similar results were performed. The data represent means ± SEM (n = 8, two independent experiments). **P < 0.01, ***P < 0.001, ns represents not significant, One-way ANOVA.