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. 2016 Nov 8;6:36365. doi: 10.1038/srep36365

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Copyright © 2016, The Author(s)

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(a,b) Sorted liver DCs from untreated SPF BALB/c mice were stimulated for 24 hr with αGalCer (200 ng), Int Bact or vehicle, respectively. Using L363, the presented glycolipids on CD1d were analyzed by flow cytometry, (a) the flow cytometric figure and (b) the statistical result were showed. (c) Staining of liver-infiltrating leukocytes from wild-type SPF C57BL/6 mice (WT) or CD1d-deficient mice with antigen-loaded CD1d dimer that constructed by loading CD1d:Ig protein with αGalCer, Int Bact, LPS or vehicle, respectively (see detail in methods). The data represent means ± SEM (n = 8, two independent experiments), ***P < 0.001, One-way ANOVA.