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. 2016 Nov 8;6:36400. doi: 10.1038/srep36400

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Immunoflurorescent staining of COL 2 in the scaffolds seeded with BMMSCs (a,b), PBMSCs (b,e), and ACCs (c,f) after 21 days of in vitro culture. Robust expression of COL 2 was present in three groups of cell-seeded scaffolds ((a–c), Scale bar = 100 μm). In a few areas ((d–f), Scale bar = 100 μm), MSCs of partial chondrogenensis produced more COL 2 than hypertrophic ACCs (g), while the percentage of MSCs secreting COL 2 is significantly less than that of ACCs (h). (Results are expressed as mean ± 95% CI, n = 3, *P < 0.001).