Table 1. Primer sequences used for real-time PCR.

	Forward primers (5′-3′)	Reverse primers (5′-3′)
COL 1	TGGCAAGAACGGAGATGACG	GCACCATCCAAACCACTGAA
COL 2	CCACGCTCAAGTCCCTCAAC	AGTCACCGCTCTTCCACTCG
COL 10	AAGTGGACCGAAAGGAGACA	TGGAAACCCATTCTCACCTC
SOX 9	AGTAC CGCACC TGCACA AC	CGCTTCTCGCTCTCGTTC AG
ACG	CGTGGTCTGGACAGGTGCTA	GGTTGGGGTAGAGGTAGACG
ALP	CGACACGGACAAGAAACCCT	TGTTGTGAGCGTAGTCCACC
GAPDH	CCATCACCATCTTCCAGGAG	GATGATGACCCTTTTGGCTC

COL 1: collagen type I; COL 2: collagen type II; COL 10: collagen type X; SOX 9: SRY-related high mobility group-box gene 9; AGC: aggrecan; ALP: alkaline phosphatase; GAPDH: glyceraldehyde-3-phosphate dehydrogenase.