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. 2016 Nov 8;7:1627. doi: 10.3389/fpls.2016.01627

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Copyright © 2016 Ezaki, Higashi, Nanba and Nishiuchi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Sensitivity test of the yeast TF carrying AL3A-4 or AL5B-2 clones on agar plates. (A) Al test on LPM agar plate (adjusted at pH4.2), (B) DM test on YNB agar plate (adjusted at pH5.7). Log phase cells of the TFs carrying either pYES3 (vector), AL3A-4 or AL5B-2 were serially diluted with sterilized water(1/5~1/3125 fold for Al test and 1~1/3125 fold for DM test) and then spotted on the agar plates. Plates were incubated at 30°C for 3 to 4 days and the sensitivity was estimated by a colony forming ability on the agar plates.