Table 1.

List of genes used for DNA methylation status analysis.

Gene	Biological function	Al response in Col-0a	Al response in TFb	Response type (Gene group)c	Methylation statusd	Sequencing region (nt)
Atlg07400	Class I Heat shock protein (HSP20)	3.52*	5.28*	I	non-ME	–187 ~+213
Atlgl7180	Glutathione transferase belonging to the tau class	6.08*	11.53*	I	non-ME	–180 ~+130
Atlgl7710	Pyridoxal phosphate phosphatase-related protein	2.66*	4.95*	I	ME	–278 ~+164
At2g34430	Photosystem 11 type I chlorophyll a/b-binding protein	0.46*	1.26*	I	ME	–160 ~+40
At3g06435	Uncharacterized protein	1.35	2.05*	I	non-ME	–388 ~+77
At3g56060	Glucose-methanol-choline (GMC) oxidoreductase family protein	0.71	0.22*	R	ME	–263 ~+209
At4gl2230	alpha/beta-Hydrolases superfamily protein	0.50	0.18*	R	ME	+123 ~+407
At4gl8940	RNA ligase/cyclic nucleotide phosphodiesterase family protein	1.08	0.07*	R	ME	+57 ~+452
At4g24310	Uncharacterized protein	0.39*	0.16*	R	ME	–172 ~+82
At4g33070	Pyruvate decarboxylase	1.01*	5.84*	I	non-ME	+1 ~+120
At5g07390(site 1)	Respiratory burst oxidase homolog A (RBOHA)	0.56	0.23*	R	non-ME	–263 ~+137
At5g07390(site2)					ME	+233 ~+593
At5g26280	TRAF-like family protein	0.39	0.15*	R	non-ME	–276 ~+109
At5g43370(site 1)	Phosphate transporter Phtl;2	0.35*	0.14*	R	ME	–228 ~ –43
At5g43370(site2)					non-ME	+296 ~+456
At5g63600	A protein similar to flavonol synthase	0.51	0.29*	R	non-ME	+736 ~+866

^a Values indicated means of the two relative holds of each gene-expression in Col-0 ecotype under +A1 condition to −Al condition in the two microarray analyses.

^b Values indicated means of the two relative holds of each gene-expression in the AvSAMSl TF line under +A1 condition to −Al condition in the two microarray analyses.

^cI, Gene group I; R, Gene group R.

^dnon-ME, no-methyktion status was Al independently kept in both lines; ME, methylation status was altered in either the tested lines under Al stress.

^*P-value of LogRatio (+AI/−AI) was less than 0.05.