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. 2016 Nov 8;6:31249. doi: 10.1038/srep31249

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Alginate samples from strains grown on PIA medium containing 300 μg/ml of Carbenicillin and 0.5% (w/v) arabinose inducer were analysed by SEC-MALLS to determine their average molecular mass and polymer length distribution (polydispersity index = PI). PI values closer to 1.0 represent a narrow M_r distribution (i.e. homogenous M_r). Strains are identified as follows: WT = PDO300(pHERD20T); ΔalgL = PDO300ΔalgL(pHERD20T); ΔalgL + algL = PDO300ΔalgL(pHERD20T:algL), and ΔalgL + algLH202A = PDO300ΔalgL(pHERD20T:algL_H202A).