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. 2016 Nov 8;6:31249. doi: 10.1038/srep31249

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Strains were grown on PIA medium supplemented with 300 μg/ml of Carbenicillin (except for PDO300ΔalgL, lane 1) and 0.05% (w/v) arabinose for 72 h at 37 °C. Envelope (E) fractions were prepared and histagged-AlgL and its potential co-interacting proteins were purified with cOmplete His-Tag Purification Resin (Roche). Envelope (E) and eluted (Elu.) fractions were run on SDS-PAGE, transferred to nitrocellulose membrane and subject to immunoblot with various antibodies (left panel: α-Alg44, α-AlgK, α-AlgG, α-AlgX and α-Histag), as described in methods section. Lane 1 = Envelope fraction of PDO300ΔalgL. Lanes 2 and 3: Envelope (E) and eluted (Elu.) fractions of PDO300ΔalgL(pHERD20T:algL), respectively. Lanes 4 and 5: Envelope (E) and eluted (Elu.) fractions of PDO300ΔalgL(pHERD20T algL_x6his), respectively. Full-length immunoblots are presented in Supplementary Fig. S4.