. 2016 Nov 8;6:31249. doi: 10.1038/srep31249

Table 1. Alginate composition as measured by H³-NMR.

Strain	F_G	F_M	F_GM/MG	F_MM	AC%
WT	0.28	0.72	0.28	0.44	70
ΔalgL	0.35	0.65	0.35	0.3	17
ΔalgL + algL	0.36	0.64	0.36	0.28	20
ΔalgL + algLH202A	0.36	0.64	0.36	0.28	19

Alginate samples, obtained from strains grown on PIA medium containing Carbenicillin (300 μg/ml) and arabinose inducer at 0.5% (w/v), were analysed by NMR to investigate their level of epimerization and O-acetylation. F_G: molar fraction of guluronate residues in alginate chain. F_M: mole fraction of mannuronate residues in alginate chain. F_GM/MG, F_MMand F_GG: molar fraction of GM/MG, MM and GG diads. AC%: degree of O-acetylation. Strains are identified as follows: WT = PDO300(pHERD20T), ΔalgL = PDO300ΔalgL(pHERD20T), ΔalgL + algL = PDO300ΔalgL(pHERD20T:algL), and ΔalgL + algLH202A = PDO300ΔalgL(pHERD20T:algL_H202A).

Table 1. Alginate composition as measured by H3-NMR.

Table 1. Alginate composition as measured by H³-NMR.