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. 2016 Nov 8;6:36695. doi: 10.1038/srep36695

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Copyright © 2016, The Author(s)

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(A) Correlation between fold change (up-regulation) of RT-qPCR (independent cDNA sets from unrelated ticks collected in the wild) and RNA-seq data on selected transcripts. The table of selected differentially expressed genes for blood-fed ticks and serum-fed ticks is shown below the graph. (B) RT-qPCR analyses of differentially expressed genes in the tick midgut dissected from I. ricinus females during and after feeding. Data were obtained from three independent cDNA sets, and normalised to elongation factor 1 (ef1α). UF-unfed; B-blood-fed; S-serum-fed; 3D, 5D, FF-indicate time periods of feeding 3 days, 5 days, 8 days (fully fed), respectively; 2 ABM and 6 ABM indicate time periods after blood meal, 2 days and 6 days, respectively. Mean and SEM are shown, n = 3 (biological replicates).