Figure 1. Identification of the T-DNA targeted gene MoPEX1.

(A) Colonies of the wild-type strain (Guy11) and the T-DNA insertional mutant (C445). Photographs were taken after incubating on complete medium (CM) at 25 °C for 10 days. (B) Barley (a) and rice (b) leaf segments were inoculated with mycelial plugs from Guy11 and C445, respectively. Photographs were taken at 5 days after inoculation. (C) Three round PCR amplifications of the genomic DNA flanking right site of the integration T-DNA in C445. (D) Position of the T-DNA insertion in C445 and the structure of the locus of the predicted gene MGG_09299. A red arrow indicates the T-DNA insertion position. The thick blue arrow represents the predicted exons. (E) MoPex1 contains two AAA-domains. Each domain contains a Walker A and a Walker B motifs, which bind and hydrolyze ATP, respectively.