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. 2016 Nov 8;6:36292. doi: 10.1038/srep36292

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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The vectors pGBKT7/pGADT7 (negative control), pGBKT7-53/pGADT7-T (positive control), pGAD-Pex1/pGBKT7, pGBK-Pex6/pGADT7, pGAD-Pex1/pGBK-Pex6 were co-transformed to the yeast strain AH109, respectively. The Leu+ and Trp+ yeast transformants were assayed for growth on SD/–Trp/–Leu/–His/–Ade medium at specified concentrations 1 × 10⁵, 1 × 10⁴, 1 × 10³, and 1 × 10² cells per 10 μl droplet.