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. 2016 Nov 8;7:1657. doi: 10.3389/fpls.2016.01657

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Copyright © 2016 Pii, Alessandrini, Dall’Osto, Guardini, Prinsi, Espen, Zamboni and Varanini.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Separation of PM H⁺-ATPase complexes by non-denaturing Deriphat-PAGE followed by SDS-PAGE. (A) Western blot analysis performed using antibodies anti-PM H⁺-ATPase. The molecular weight of each protein marker (ECL Plex Fluorescent Rainbow Markers) was reported on the right side. (B) Densitometric analysis of PM H⁺-ATPase. Data are expressed as ratio of corresponding 0 h sample considering the sum of all three forms (signals were quantified using PDQuest^TM 2-D Analysis Software, Bio-Rad). Data are the means ± SE, n = 3.