Table 1. Positions of transposon insertion in the genome of P. furiosus COM1, with corresponding genes, proteins and their annotation.
| Mutant strain | Transposon insertion coordinates in COM1 | Gene* | Protein | Annotation |
|---|---|---|---|---|
| 1 | 116279 | PF0300 | AFN03089.1 | hypothetical protein GTP: adenosyl-cobinamide-phosphate guanylyltransferase |
| 2 | 869063 | PF1118 | AFN03909.1 | CRISPR-associated protein Cas1 |
| 3 | 216315 | PF0407 | AFN03198.1 | hypothetical protein |
| 4 | 1200285 | PF1504 | AFN04287.1 | large helicase-like protein COG1201 Lhr-like helicases |
| 5 | 1770485 | PF1756 | AFN04884.1 | hypothetical protein COG0393 Uncharacterized conserved protein |
| 6 | 775060 | PF1016 | AFN03812.1 | 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase |
| 7 | 482399 | PF0669 | AFN03462.1 | hypothetical protein COG0454 Histone acetyltransferase HPA2 and related acetyltransferases |
| 8 | 811244 | PF1055 | AFN03851.1 | threonine synthase |
| 9 | 1526411 | PF2033 | AFN04610.1 | hypothetical protein predicted Fe-S oxidoreductase of SAM family |
| 10 | 737427 | PF0978 | AFN03773.1 | hypothetical protein COG1800 Predicted transglutaminase-like proteases |
| 11 | 982898 | PF1245 | AFN04032.1 | d-nopaline dehydrogenase COG0446 Uncharacterized NAD(FAD)-dependent dehydrogenases |
| 12 | 1489490 | PF0006 | AFN04032.1 | ABC transporter COG1131 ABC-type multidrug transport system, ATPase component |
*The corresponding gene loci numbers from the wild-type DSM 3638 strain are presented for convenience.