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. 2016 Oct 21;26(11):1242–1254. doi: 10.1038/cr.2016.117

Figure 7.

Figure 7

Creating OsMiMe. (A) Crossing scheme. OsMiMe mutant was created first by crossing B01997 plants heterozygous for the Osrec8 mutation with AQUG12 plants heterozygous for the pair1 mutation (step 1). 1/4 of F1 were double heterozygous plants and were in turn crossed with AMBA12 plants heterozygous for the Ososd1 mutation to produce 1/8 of F1 triple heterozygous plants (Osrec8+/−; pair1+/−; Ososd1+/−) (step 2). These plants were selfed (step 3) and the progeny were genotyped to identify 1/64 triple homozygous OsMiMe plants and relevant control segregants. HW, Hwayoung; NB, Nipponbare; He, heterozygous. (B) Fertility in MiMe and related mutants. The histogram represents the percentage of flowers giving a seed by self-fertilization. Numbers of observed flowers and seeds are indicated. Errors bars correspond to the SD for 4-7 plants.