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. 2016 Oct 21;26(11):1182–1196. doi: 10.1038/cr.2016.118

Figure 6.

Figure 6

Neuropeptide regulates cell non-autonomous UPRmt signaling. (A) Cell non-autonomous UPRmt is suppressed in egl-3 mutants. Peripheral expression of hsp-6p::GFP induced by neuronal knockout of spg-7 in each strain is quantified. n ≥ 30. (B) qRT-PCR measures transcript levels of egl-3 and egl-21 in rab-3p::Cas9+u6p::spg-7-sg; hsp-6p::GFP worms. Fold changes are compared with the control hsp-6p::GFP worms. (C) hsp-6p::GFP induction in rab-3p::Cas9+u6p::spg-7-sg animals with the neural deletion of each indicated neuropeptide genes is quantified. The numbers of percentage under 30% are marked in red. (D) Individual deletion of six indicated neuropeptide genes in the nervous system suppresses the peripheral induction of hsp-6p::GFP. n ≥ 20. (E) Transcript levels of six indicated neuropeptide genes in rab-3p::Cas9+u6p::spg-7-sg; hsp-6p::GFP worms are measured by qRT-PCR. Fold changes are compared with the control hsp-6p::GFP worms. Error bars indicate mean ± SE. A Student's t-test is used to assess significance: *P < 0.05, **P < 0.01,***P < 0.001.