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. 2016 Nov 8;6:36303. doi: 10.1038/srep36303

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Each SG clone was allowed to form salisphere-like cell structure followed by salivary epithelial cell differentiation as described in the methods. Salivary epithelial differentiation was evaluated by immunofluorescence staining for AQP5 and α-amylase. Scale bars represent 20 μm. (b) Expression of salivary epithelial differentiation-associated molecular markers including AMY1A, AQP5, and BHLHA15 was examined by semi-quantitative RT-PCR. (c) To assess the multiple epithelial differentiation potential of the SG clones, each clonal population was subject to in vitro hepatogenic differentiation. The differentiation was evaluated by PAS staining. (d) Expression of hepatocyte differentiation markers including ALB and TJP1 was analyzed by RT-PCR.