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. 2016 Nov 8;6:36303. doi: 10.1038/srep36303

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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As a representative GSC, clone C1 was subject to salivary epithelial differentiation for up to 14 days. RNA samples were isolated at the indicated time points. Q-PCR was conducted to examine the temporal expression patterns of genes including AMY, AQP5, CK5, CD24, CD90, CD49f, LGR5, and LGR6. Results are presented as the means ± SEM. One-way ANOVA and Tukey’s post hoc multiple comparison test were performed. *, vs. Day 0; #, vs. Day 1; $, vs. Day 7. (*p < 0.05, **p < 0.01, ***p < 0.001, ^#p < 0.05, ^##p < 0.01, ^###p < 0.001, ^$$p < 0.01).