Figure 4. LRBA plays a pivotal role in induction of MIP-1α and cytolytic function.

(A) The NK cells were isolated from WT or LRBA-null spleens by MACS and co-incubated with PD1.6 or RMA-Rae1 tumor cells in a 96-well multi-test plate at 37 °C, 5% CO₂ for 20 hr. Cell-free supernatant was harvested and MIP-1α production measured by ELISA assay. (B) The multi-test plate was used to determine the number of Granzyme B secreting NK cells by ELISpot assay. The data in (A) and (B) are representative of three independent experiments.***p < 0.001,****p < 0.0001.