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. 2016 Nov 8;6:36039. doi: 10.1038/srep36039

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Copyright © 2016, The Author(s)

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(a) Breeding scheme to generate triplegenic mice. (b) Coronal brain slices containing raphe nucleus show co-localization of ChR2 (green) with 5-HT (red) in both DR and MnR. (c) Representative coronal sections of LHA from triplegenic mice. Sections were labelled with antibody for GFP (green), orexin (blue), and serotonin transporter, HTT (red). LM, lower magnification. Arrowheads indicate 5-HT nerve endings and arrows indicate orexinergic nerve terminals. These were in close apposition in the LHA.