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. 2016 Nov 8;6:36039. doi: 10.1038/srep36039

Figure 4. Activation of 5-HT nerve endings induced an outward current and decreased the firing rate of orexin neurons in triplegenic mice.

Figure 4

(a–c) Outward currents recorded from orexin neurons at −60 mVhold in the presence of 1 μM extracellular TTX were abolished in the presence of the 5-HT1A antagonist, WAY100635. Representative trace showing the light evoked outward current (a), which was blocked by WAY100635 (b). (c) Combined bar and scatter plot summarizing the data in a and b; vehicle, n = 12; WAY100635, n = 21; vehicle vs WAY100653, p = 3.1 × 10−19 (light), 0.46 (pre), 0.58 (post). (d,e) Spontaneous firing of orexin neurons was recorded by loose cell-attached recording. (d) Representative trace showing the decreased firing induced by light. (e) Combined bar and scatter plot summarizing data in d (n = 14; p = 0.004 vs pre; and 5 × 10−4 vs post). **p < 0.01, ***p < 0.001. p values were calculated by one-way ANOVA followed by a post hoc Fisher’s LSD test.