Figure 4. Ligand interaction with CCR5 StaR reconstituted in a POPC membrane monitored by PWR.

The ligand maraviroc was incrementally added to the proteolipid membrane and the shifts in the resonance minimum position followed. The data was fitted with a hyperbolic binding equation that describes total binding to a single site in the receptor (more details in Materials and Methods). Dissociation constants obtained from the data are presented in Table 2.